FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405289

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405289
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3762998
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50560.1343609536863958No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCTAG11700.019.1860711
GTGCTAG8750.017.3661351
GCGCATA1200.001467641617.2674641
CGTAACC1602.9694726E-517.2389833
ATAAGCG2451.33040885E-817.1434856
GTCCTAG9650.016.820121
GTACGCG1653.8121227E-516.7442071
TCTAGAC7350.016.418083
GTATTAG5900.016.3894561
CTTAGAC7650.016.2249243
TCTAGGC10650.016.1868383
GTACTAG3651.8189894E-1216.084761
TAAGCGT3151.0713848E-915.59444957
TACTAGG5100.015.5623432
GTCCTAA7800.015.4964411
GTACTAA6050.015.4122831
GCTAGAC6050.015.3868623
TTATACG1808.1902064E-515.3119324
GTCTTAG7750.015.1508071
GTATTAA9150.015.0972371
Produced by FastQC (version 0.11.5)