FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405290

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405290
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3826033
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG6450.018.684461
GCGCTAA1351.6018805E-417.854041
GTATAAG7200.017.2163961
CCTATAC5600.016.6023923
GTCTTAA8850.015.9519151
GTCCTAG8000.015.9251661
GTTCTAG9550.015.8643231
GTATTAT9500.015.585371
GGTCTAG7850.015.35220051
GTCCTAA8350.015.2576441
GTACTAG3757.094059E-1114.6913231
TATACGT1709.254287E-414.1789495
GTATAGA7300.014.1504621
GTCCTAC7600.014.0449541
GGTCTAA5900.014.0065591
CTAGTAC3951.6007107E-1013.9481893
GCTAGAC5200.013.9062773
GTATATG8800.013.69485951
GTGCTAG8500.013.3680251
GTATAGG6050.013.0901531
Produced by FastQC (version 0.11.5)