FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405293

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405293
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3736375
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG46580.12466628751129102No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG9300.021.8711221
GTACTAA6500.018.5633641
GGTCTAG6700.018.0092331
GTCTTAG7350.017.8237151
GCGTTAA1452.7396914E-416.6430151
GTATAGG5550.016.1503661
CGTCTAA1503.547453E-416.088251
GTGCTAA7800.015.9114561
GTATACG1554.552714E-415.5692731
TATACGA1554.570826E-415.5611442
GTACTAG3552.910383E-1115.5379471
GTATTAA9900.015.3221421
TGTACCG3603.6379788E-1115.3090185
GCGATCG1851.0344367E-414.898252537
GCTCTAG9850.014.6999231
GTACCGA3051.4988473E-814.6814036
GTGCTAG7800.014.5855021
GTCCTAA7850.014.49259951
TCTAGGC10950.014.1561673
TATACTG10950.014.1555995
Produced by FastQC (version 0.11.5)