FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405294

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405294
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10824704
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG12750.018.3326741
GTCTTAG22100.016.0203511
GTGCTAG26200.015.218921
GTATTAG19250.014.9994611
GCTCTAG32850.014.9633121
GTCCTAA21100.014.33597851
GTATTAA27650.013.9235511
GTATAAG20050.013.886661
TAGTGCG5650.013.70462457
GTATTAT30150.013.5670921
GTCCTAT21550.013.3985911
GTACTAA19200.013.2482221
GTTCTAA28850.013.2252611
GGTCTAG21850.013.2146291
GTGCTAA25350.013.0172841
GTACATA27250.012.9926531
CTTAGAC20650.012.8172183
GTCCTAG26300.012.6777771
TCTATAC20400.012.6372973
GTACAGG47300.012.5722241
Produced by FastQC (version 0.11.5)