FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405295

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405295
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10824704
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG26400.016.1807371
TACGCGA2351.8716128E-716.1103652
GTCCTAA22400.015.840531
GTACGCG3703.6379788E-1215.8280611
GTACTAA19400.015.8040651
TCTATAC22600.014.6089843
GTATTAA26750.014.552421
GTACATA26550.014.4025361
CTATACT22650.014.266724
GGTCTAG18900.014.21717551
GTCTTAG23600.014.1592491
GTATAGG16750.013.9853921
GTACTAG11650.013.8980051
ATAGTAC16150.013.8419743
GTATTAG19100.013.7075791
GTTCTAA30800.013.5336571
GTGCTAG23700.013.5180841
CGAATCG4609.094947E-1213.48534745
ACGCGAC2851.6718332E-613.2740983
TAGTGCG5600.013.19757557
Produced by FastQC (version 0.11.5)