FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405298

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405298
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10677922
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG12700.017.3232691
GTTCTAG27800.016.4460071
GTATTAG17350.016.2468051
CGAATCG5250.015.7119579
GTATTAA27350.015.3340231
GTCTTAG20850.014.8385021
GGTCTAG18800.014.2623261
GTCCTAG25350.014.1029191
GTCCTAA21800.014.0341831
GTACTAA18150.014.0155041
GTGCTAG24700.013.778181
TCTAGAC22250.013.7502813
GTTCTAA28150.013.4328411
GTATAGG17150.013.2291961
GTATATG27950.013.159991
CTAAGCG7850.013.137214
GTATTAT27500.013.125331
CGATCGT4204.3837645E-1013.09697838
TCTGTAC45100.013.0338033
GCGCTAG4255.293259E-1012.9415021
Produced by FastQC (version 0.11.5)