FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405299

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405299
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10677922
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG10750.016.0055521
GTCCTAG23950.015.9487471
GTTCTAA29500.014.8146311
CTAGACC19350.014.3893464
GTGCTAG23900.014.2543161
GTATTAA29050.014.2149131
CTATACT21400.014.1353294
GTTCTAG28250.014.1302121
GTCCTAA20900.013.6659841
GCCCTAG27200.013.5370481
GTCTATA18850.013.5091951
TAGTGCG5500.013.43224157
GTACTAA17950.013.4196971
GTACAGG46200.013.4072481
GTATATA34750.013.3686651
GCTCTAG30000.013.3059491
GTATAAG19200.013.2629341
TCTAGAC22500.013.1389633
CGAATCG5003.6379788E-1213.0616569
TCTATAC18750.012.83340553
Produced by FastQC (version 0.11.5)