FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405300

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405300
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10457160
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG11600.017.1862551
GTATTAG19250.016.2488231
GTGCTAG23400.015.7173431
GTACTAA17850.015.2124821
GCGCTAG3654.7293724E-1115.0674011
CGATCGT4800.015.0427238
GTATAGG16500.014.7905951
GTCTTAG24000.014.7515351
GTATTAA28000.014.6083161
GTGCTAA22850.014.5913931
GCTCTAG29900.014.48473551
GTCTTAA26450.014.2948061
GTTCTAG26900.014.0556731
GTTCTAA27550.013.9735791
GTCCTAG28100.013.9447181
GTATATA31150.013.7931411
CTAGACT20450.013.7824894
TCTGTAC42000.013.4215183
GCGATCG4651.0913936E-1113.30835137
TCTAGAC22300.013.1015033
Produced by FastQC (version 0.11.5)