FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405301

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405301
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10457160
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG11650.018.0150031
GTCCTAG24350.016.3903961
GTATTAG19650.015.7583381
GGTCTAG19750.015.5043441
GTACTAA20200.015.1589491
GCTCTAG30600.015.066551
GTATAAG19300.014.0831631
GTCTTAA26950.013.7878141
GTATTAA27800.013.737531
GTACTAT18050.013.7241591
GTGCTAG25150.013.6802011
GTCTTAG22150.013.6690841
GTTCTAA28850.013.5953231
GGTCTAA18350.013.4997861
GTCCTAA22550.013.4266171
ATAAGCG7650.013.30256856
GTTCTAT25750.013.0942111
GTGTTAG26850.012.94218351
GTATATG27750.012.7704061
GTATTAT28100.012.4889031
Produced by FastQC (version 0.11.5)