FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405305

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405305
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12130707
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG162390.1338668883849886No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG25450.016.9332541
GTATTAA34950.016.6708491
GTACTAG15000.016.548531
GTCCTAG31050.016.2109871
GTGCTAG32150.015.4418641
GTCTTAG27900.015.1991441
GGTCTAG23600.014.1702621
GTACTAA20950.013.9879191
GTTCTAG37900.013.9177941
GTCCTAA28600.013.6216771
GTTCTAA35550.013.5770881
GTATTAT35050.013.4756821
TACGCGA2052.473728E-413.4453152
GTATATA46100.013.1622441
GCCCTAG31150.013.059971
GTACAGG52400.013.027231
CTATACT25550.012.9342864
GTACTAC18450.012.8935031
GTACTAT22350.012.8032071
GTATAAG25450.012.7338081
Produced by FastQC (version 0.11.5)