FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405306

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405306
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12215559
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG24600.017.1984441
GTCCTAG32450.016.0059471
GTACTAG15100.015.2621951
GTCCTAA25450.015.0021791
GTATTAA34300.014.9420881
GTATAGG21050.014.7065071
GTACTAA22500.014.3702551
GTGCTAG31200.014.0012971
GTCTTAG27600.013.833531
GTTCTAA34450.013.5790331
GTATATA43100.013.4076031
TCTAGAC30800.013.066353
GTTCTAG38150.012.8030361
CTATACT30050.012.5912074
CTAGACT29000.012.5726564
GTATTAT35650.012.5430451
GGTCTAG24350.012.4309071
CGAATCG4752.2191671E-1012.34026845
GCCCTAG31750.012.2420431
ATAGTAC21150.012.1974773
Produced by FastQC (version 0.11.5)