FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405307

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405307
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12215559
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG34550.016.4328481
GTATTAG25800.015.6042571
GTGCTAG33650.015.3385081
GTCTTAG27900.015.0463971
GCTCTAG33900.014.9208841
GCGTTAG4700.014.6422921
GTACTAG15750.014.6376451
GTATATA44050.014.3730471
GTATTAT35700.014.2649561
GTTCTAA36050.014.0310131
GGTCTAA24600.013.847681
GGTCTAG25250.013.7637541
GTACTAA23250.013.6157571
GTTCTAG36700.013.1261971
GTGTTAG33900.012.6878271
GTCCTAA28100.012.6126921
GTATTAA34450.012.5851461
GTCTATA22850.012.4988151
GGCTTAG30700.012.4411791
GCCCTAG31700.012.1572591
Produced by FastQC (version 0.11.5)