FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405308

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405308
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12206132
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG24800.017.197951
GTCCTAG31200.016.5364911
GTACTAA24450.015.7559951
GTACTAG17300.015.1103371
GTATTAA34800.014.9246581
GTATAGG20300.014.74111
GTTCTAG34650.014.6914661
GTCCTAA27950.014.3982831
GCCCTAG30450.014.3457461
GGTCTAG23450.013.9343741
GTGCTAG31900.013.9093131
TCTATAC27450.013.909013
GCTCTAG37500.013.6666371
GTACTAT23250.013.4624811
GTGCTAA29850.013.0208931
GTACATA35900.012.9343911
GTGTAGG40700.012.9301541
CTAGACT27700.012.6658974
GTCTATA24200.012.6497321
CTATACT28300.012.6404494
Produced by FastQC (version 0.11.5)