FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405309

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405309
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12206132
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG150940.12365915754474882No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG14950.016.5704611
GTATTAG26750.016.3351541
GTGCTAG32650.014.4371241
GTATATA46550.014.1913881
GGTCTAA24500.014.043561
GTACTAA21500.013.922721
GTTCTAG35700.013.8783421
GTCTTAA34700.013.6833651
TCTAGAC30400.013.5789863
GTCCTAG31950.013.4611591
GTGCTAA27450.013.4117291
GTTCTAA37500.013.3956841
GTATTAA35550.013.3561961
GTCCTAA27700.013.042261
GTCTTAG28550.013.0155031
GGTCTAG26450.012.8781311
CTATACT28800.012.6610444
GTATAAG24450.012.5243291
GTCCTAC25100.012.4741511
GTCTTAT30900.012.47104551
Produced by FastQC (version 0.11.5)