FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405310

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405310
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12388659
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAA23350.015.6044621
GTATTAA33750.015.5828711
GTACTAG13050.015.5407271
GTATTAG24150.015.3722151
GTCCTAG30350.015.1766491
GTGCTAG30050.014.8706061
GTCTTAA32350.014.1321141
GTATAGG19650.013.6446321
GGTCTAG23450.013.485741
GTATAAG23050.013.4215111
GTCCTAA27750.013.377981
GTATATA39600.013.1940521
GTATATG35000.013.1603221
GTTCTAG35200.012.9878961
GTACTAT22400.012.4298761
GGTCTAA22700.012.4170311
ATAGTAC20600.012.3479353
GTACAGG53600.012.1848061
GTATAAA44400.012.1547621
GCTCTAG33500.012.107851
Produced by FastQC (version 0.11.5)