FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405315

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405315
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12287151
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG14900.020.3054711
GTCCTAA26150.016.5659181
GTATTAG21650.015.72149851
GTACTAA22550.015.7038921
GTCCTAG32000.015.1490641
GCGATCG4400.014.84724437
CGATCGT4700.014.63271338
GTTCTAA35550.014.4099771
GTCTTAA30550.013.9549111
GTATATA41250.013.9190361
GTCTTAG25250.013.8885031
GTATAGA28100.013.7034011
GTATACG4851.8189894E-1213.4687871
GTATTAA32950.013.2514961
ATAGTAC20700.013.115163
GTGCTAG30350.013.0273431
GTATATG35200.012.9904921
GTCCTAT26250.012.8355191
GTTCTAG35200.012.7951461
GTACTAT21500.012.7928751
Produced by FastQC (version 0.11.5)