FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405316

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405316
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12247994
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG13900.017.3061031
GTATTAG23000.016.4354231
GTCCTAG30900.015.4586791
GTATTAA31700.014.7433331
GTTCTAG33100.014.6388571
GTACTAA22600.014.1413381
GTACAGG55650.014.0176991
GTTCTAA32800.013.3059511
GGATTAG25850.013.0281151
CTGTACT60750.013.0109264
GTATAGG21200.012.9679161
GTATTGG28900.012.96118451
GTCTTAA31100.012.9283021
GTCCTAT27350.012.8161881
GTATATG34400.012.7869671
GTATATA44050.012.716211
TCTATAC24900.012.6973583
TATACTG33100.012.6665545
ATAGTAC19600.012.4487913
CTAGACT25700.012.435884
Produced by FastQC (version 0.11.5)