FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405317

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405317
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12247994
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG153100.12500006123451726No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG14150.017.4925841
GTGCTAG30350.016.3110431
GTCCTAG31750.016.13321
GTATAGG18750.015.9512941
GTATTAG24550.015.403491
GTCTTAG27850.014.6892531
GTTCTAA31300.014.6078161
GTATTAA31450.014.2102151
GTTCTAG34700.014.1672161
TATACTG32700.013.6635515
GTGCTAA29950.013.5445051
GGTCTAG24250.013.3258231
GTACTAA23300.013.1314311
GTCTTAA32500.013.1164491
GTATATG32150.013.0453821
GTCCTAA26500.012.9727511
GGTCTAA22900.012.9104361
CGAATCG4551.1459633E-1012.8410929
CTAGTAC13850.012.655613
TTATACT37600.012.5225744
Produced by FastQC (version 0.11.5)