FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14092810
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG19100.017.536771
GTATTAG28050.017.3579121
GTCCTAG35650.015.9821661
GTCTTAG33700.015.26748851
GTGCTAG37500.015.0095471
GTATAGG22350.014.986681
GTTCTAG40450.014.5978481
GTATTAA44100.014.4858641
GTTCTAA42850.014.3443361
GTACTAT26100.014.1564551
GTACTAA26750.014.0706431
GTCCTAA34000.013.7109041
GTATATA50050.013.5916871
GCCCTAG34200.013.5297551
GGTCTAG25000.012.8455931
GCTCTAG40700.012.81131651
CTAGTAC16500.012.74573
GTATATG43050.012.673461
TCTAACC30400.012.5883043
GTACAGG59450.012.43005851
Produced by FastQC (version 0.11.5)