FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405320

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405320
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14057833
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG27600.016.0940761
CTATACT32500.015.4689594
GTACTAG18100.015.0291341
GTTCTAG37950.014.9712341
GTCCTAG34300.014.9581391
GCGATCG5300.014.94585837
GTGCTAG35550.014.9164861
CGATCGT5300.014.29720838
GTATATA53050.013.890371
GTACTAA26200.013.6683931
GTATTAA39500.013.5991871
GTATAGG22700.013.5004921
TCTATAC31150.013.3755853
GTCTTAG30850.013.2824221
CGAATCG5250.013.1171419
GTCCTAT30150.013.0197591
GTATTAC24750.012.7996491
TATACTG39750.012.5609955
ACTATAC24700.012.5467433
GTATAAG27000.012.4982081
Produced by FastQC (version 0.11.5)