FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405321

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405321
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14057833
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG35450.018.7063881
GTACTAA26950.016.2760831
GTTCTAG40700.016.0388161
GTATTAG28450.015.6607441
GGTCTAG26250.015.1312041
GTGCTAG36750.014.9432371
GTCTTAG32650.014.9156051
GTATACG5150.014.7543611
GTATTAA43150.014.4077661
GTACTAG15450.014.3072581
GTCCTAA30450.014.2918411
TCTAGAC31500.014.0095463
GCTCTAG41500.013.6489961
GTATAGG21150.013.3908591
GGTCTAA27300.013.1575681
GTATATA52150.013.0471861
GTACTAT26050.012.7282431
GTATAAG29050.012.7216241
CTAGACC27850.012.6174134
TGTACTG52450.012.6067375
Produced by FastQC (version 0.11.5)