FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405322

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405322
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13761437
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG17050.017.1614341
GTATTAG28550.016.518611
GTCCTAG34650.014.9020841
GCTCTAG41650.014.6290891
GTTCTAG37700.014.6095771
GTCTTAG30200.014.5902271
GTATTAA41400.014.0522351
TATACTG38650.013.8052035
ATAGTAC25200.013.6602443
GTACAGG62550.013.6484521
GGTCTAG28400.013.575591
GTATAGG23150.013.382911
TCTAGAC29600.013.3741183
GTCCTAA29400.013.348011
GTACTAA24050.013.3114981
GTGCTAG33900.012.9977841
GTTCTAA39200.012.9967461
GTATATA49950.012.8184791
GTGTTAG36900.012.7806591
CTAGACT30750.012.7624
Produced by FastQC (version 0.11.5)