FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13660611
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG17600.019.7587361
GGTCTAG26950.016.0976411
GTATAGG22250.015.7841291
GTATTAG27650.015.3165321
GTGCTAG35350.015.2918731
GTTCTAG37750.015.2317561
GTCCTAG35850.014.5983881
TTGCGCG5350.014.15737612
GTACTAA26550.014.1355411
GTCTTAA37300.014.1231991
GTATTAA37900.013.9904591
GTATAAG27750.013.6483511
GGTCTAA27000.013.6449041
GTTCTAA38050.013.48286151
GTATAGA30550.013.4117741
GTACAGG56650.013.3105091
TATACTG41250.013.2717565
GTCTTAG31700.013.1424581
GCTCTAG39300.013.0540181
GTATATA51450.012.8489111
Produced by FastQC (version 0.11.5)