FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405325

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405325
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13660611
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG16750.019.7517051
GTATTAG25800.017.3648821
GTCCTAG33900.016.2655411
GTTCTAG39500.015.9662231
GTGCTAG35650.015.5637611
GGTCTAG26300.013.7588391
GTCTTAG31800.013.6549971
GTATTAA39950.013.629771
GTACTAC20350.013.5479561
GTACTAA27400.013.4580281
GTACAGG60500.013.4432681
GCTCTAG41650.013.238941
GTCTTAA37300.013.1198151
GTATACG5800.013.0720261
GTGCTAA33800.012.948971
ATAGTAC26350.012.9377633
GTATAGG21100.012.9030641
CTATACT31700.012.8160054
GTTCTAA40050.012.7352481
CCTATAC20350.012.6911653
Produced by FastQC (version 0.11.5)