FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405326

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405326
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11686962
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG21950.018.9438171
TAGTGCG6250.015.76936157
GTCCTAG33300.015.0669481
GTACTAG12700.014.882491
GTATATA38850.014.4182721
GTACTAA21750.014.378011
GTACTAT22450.013.9296981
GTATTAA31100.013.9228071
GTATAGG18800.013.7094511
GTTCTAA31300.013.2848821
GCTCTAG34200.013.163211
CGATCGT4751.6370905E-1113.02805338
TTATACT35400.012.91109854
GTTCTAG33600.012.8868841
GTGCTAG29000.012.5610091
ATAAGCG7600.012.46971756
CTATACC21350.012.3938894
CTGTACT56050.012.2622224
GTCTTAG25800.012.2541941
GATCTAG23050.012.2253011
Produced by FastQC (version 0.11.5)