FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405327

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405327
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11686962
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG24450.017.8872571
GTCCTAG30600.016.543021
GTCTTAG25800.015.6165491
GTGCTAG27450.015.3051071
GTTCTAG33150.015.0627181
GTTCTAA32200.014.5446051
GTACTAA19100.014.4236611
GTATATG33200.013.6916181
GTCCTAT25550.013.6128591
GTACTAT20350.013.1992451
GTATTAA31400.013.1604421
GTCCTAA24100.013.1458821
GCTCTAG35500.012.9985631
GTACTAG14750.012.8407261
GGTCTAG24400.012.7019841
GTATAGA24050.012.6004631
TATACTG33500.012.4143575
CCTCTAG48300.012.3343951
GGTCTAA21850.012.2931181
GTATAGG16700.012.1661851