FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405328

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405328
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11543544
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG21950.018.0031381
GTCCTAG28150.016.4793551
CTATACT25600.014.8993694
GTCTTAG26600.014.4684261
GCTCTAG33550.014.3390511
GTTCTAA30650.014.2382991
GTACTAA20550.014.0459891
GTATTAA30600.013.9246781
TATACTG33450.013.8681575
GTTCTAG32400.013.5753131
GTATAGG18750.013.5617371
GTACTAG14050.013.4515161
GTACTAT18900.012.7268551
CGATCGT4601.364242E-1012.70425238
GTCTTAA30850.012.5865921
GTATAAG23800.012.5610861
GTACTAC17900.012.4780061
TTATACT36150.012.45225054
GTATTAT31750.012.4462641
GTGCTAG29650.012.2847421
Produced by FastQC (version 0.11.5)