FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405329

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405329
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11543544
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG153000.13254161806807335No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG30100.016.5890431
GTATTAG21150.016.4448851
GTACTAG14400.015.7834261
GTCCTAA26950.014.6946381
GTTCTAG32600.014.2605411
GTCTTAA31050.014.1960721
GTGCTAG28550.014.1123591
GTATTAA32900.014.0258361
TTGCGCG5200.013.87828112
GTACTAA21850.013.8691891
GTATAAG22550.013.7440831
CTAGACT25600.013.5655764
GGTCTAG23400.013.5391631
GCCCTAG30200.013.1132621
TAGTGCG6450.013.09158857
GTATAGT20300.012.8925081
GTATATA39400.012.6733561
GTCTTAG25950.012.6068341
GTTCTAA29800.012.4803671
GCTCTAG34300.012.2485751
Produced by FastQC (version 0.11.5)