FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11594637
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG20050.019.3654251
GTACTAG13450.017.116521
GTCCTAG30900.016.902391
TACGCGA2353.3611868E-614.62186052
GTACTAA21200.014.5871221
GTTCTAG32100.014.4507951
GTATTAA31050.014.2754921
GTGCTAG30950.013.8775351
GCTCTAG33700.013.458821
ATTAGAC21000.013.4174143
GTTCTAA31450.013.3291411
GTATAGG19400.013.2838051
GTCTTAG27200.013.1379441
GTCCTAA25450.013.0962491
GTATATA39550.013.03191
GCCCTAA24900.012.9715391
CCCCTAG33650.012.6619211
GTATTAT31500.012.5444121
GGTTTAG27000.012.4716891
CTAGACT26550.012.4245584
Produced by FastQC (version 0.11.5)