FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405333

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405333
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11594637
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG145570.12554942427261845No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG29850.017.0414751
GTGCTAG28550.016.1320111
GTTCTAG32250.015.87984851
GTCCTAA25600.015.5742751
TATACTG33100.015.572745
GTATAGG18450.015.0896251
GTATTAA31250.015.0681631
GTTCTAA33150.014.9303071
GTATTAG21650.014.6056111
GCGATCG5650.014.60150337
GGTCTAA22600.014.4479071
CGATCGT5700.013.87167738
GTCTTAG28050.013.6012831
GTATATA38200.013.4963921
GTCCTAT22700.013.47578051
TAGTGCG5750.013.46596157
GCCCTAG28950.013.4158961
TCTATAC24900.013.248313
CTATACT23400.013.0695594
GTCTTAA29250.012.9257751
Produced by FastQC (version 0.11.5)