FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12486864
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG14100.018.8110391
GTATTAG23300.017.4448491
GTCCTAG30600.015.9848331
GTATTAA33550.015.7086941
GTTCTAG32650.015.2976951
GTACTAA21100.014.6926871
GTCTTAG28100.014.0971941
GCTCTAG38900.013.9024461
GTATAAG23900.013.8361251
GTGCTAG31900.013.4977221
GGTCTAG25550.013.48187351
TCGCGTA1800.001424406213.39225213
GTCCTAA24350.013.2975021
TCTAGAC26900.013.0613283
TATACTG34600.012.9422095
GCCCTAG31000.012.8895411
ATTAGAC22350.012.7920453
GTCTTAA29800.012.7150351
GTATATA39900.012.69070151
CGATCGT6300.012.57619838
Produced by FastQC (version 0.11.5)