FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405335

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405335
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12486864
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG30700.016.9786571
GTACTAG14200.016.5304761
GTGCTAG28650.015.1813611
GTTCTAG34450.014.8298671
GTATAGG17500.014.7940821
GCGCTAG4700.014.6891591
GTATTAA33800.014.6044141
GTCTTAG28750.014.5282171
GTACTAA21850.014.3765531
GTCCTAA28500.013.5655661
TCTATAC25800.013.4900113
GTTCTAA35100.013.4734331
CGATCGT4901.8189894E-1213.35724238
ATAGTAC19550.013.2198083
GTATTAG22300.013.1576661
CTATACT25450.012.993114
GTATATA39050.012.8177491
GTATAAG22950.012.7850091
GGTCTAG23250.012.7685131
GGTCTAA22450.012.7622291
Produced by FastQC (version 0.11.5)