FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405336

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405336
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12396159
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG22300.016.6801361
GTTCTAG34900.015.6910641
GTACTAA20850.015.5275341
GTATATA37450.014.8985471
GTCTATA22300.014.054561
GTCCTAG29950.014.029551
GTTCTAA34450.013.8965261
GTCTTAG30300.013.8674931
GGTCTAG23750.013.7765581
GCTCTAG33300.013.6524451
TCTAGAC26400.013.56782153
CCTATAC18600.013.3321523
GTACTAG15100.013.2291451
GTATTAA34750.012.9836621
GTACAGG56650.012.9497191
GTATAAG23500.012.89720251
GTATTAT31350.012.85372451
GTGTTAG28800.012.5567571
TCTGTAC50750.012.4871263
GTGCTAA27600.012.3539771
Produced by FastQC (version 0.11.5)