FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405337

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405337
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12396159
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG15550.017.7573761
GGTCTAG23100.016.5855621
GTTCTAG33150.016.0345341
GTATTAG22500.015.80061151
GTCCTAA23800.015.5176521
GTGCTAG30900.015.3031651
GTCCTAG28950.015.1416881
TCTAGAC26000.015.1101343
GCTCTAG38050.014.7860481
GTATAGG19350.014.4485151
GTACTAA21400.013.8708741
GTACTAT21850.013.42723751
GTCTATA24200.013.40700151
GTATAAG21700.013.3609931
GTACAGG56150.013.2162391
GTGTTAG29350.012.818511
CTATACC22850.012.8155754
GTATTAA32950.012.7797861
TCTAGGC39650.012.6895573
GTCTTAA32150.012.6683541
Produced by FastQC (version 0.11.5)