FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405338

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405338
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12233251
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG14100.016.1253661
GTCCTAG29100.015.6266421
GTATATA38650.015.4198931
GTCCTAA25800.014.821361
GTATTAG22450.014.4243511
GTATTAA30600.014.29772951
GTTCTAG32700.013.8009281
GTCTTAG26500.013.519861
GTTCTAA34950.013.4052981
GGTCTAG23400.013.2498641
TCTAGAC27000.013.1416343
TCTGTAC49100.013.1200653
CTATACT25050.013.0645754
GCGATCG5650.012.80429337
GTACATA32500.012.719871
GCTCTAG34450.012.5998721
CGATCGT5501.8189894E-1212.52806638
GTACTAT19050.012.4778241
GTACAAG43800.012.4270421
GTCTATA22200.012.4142871
Produced by FastQC (version 0.11.5)