FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405341

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405341
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12042070
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG12500.018.7575241
GTATTAG22450.018.1235081
GTCCTAG30350.016.8143331
GTTCTAG32900.016.3495331
GTCCTAA24450.015.0897321
GTATACG3907.2759576E-1215.0300671
GTGCTAG30150.014.9816851
GGTCTAG22600.014.7992571
GTATTAA34700.014.7064861
GTACTAA21800.014.0770011
GTGCTAA28750.013.91223051
TACGCGA2751.0917975E-613.7863962
GTGTTAG27050.013.2569211
GTATATA38850.013.1355221
GTCTTAG25450.013.0064891
GCTCTAG35550.012.9969611
CGAATCG5650.012.8052089
CCTATAC19000.012.69551853
CTAGACC22900.012.3388184
GTACATA29650.012.3270121
Produced by FastQC (version 0.11.5)