FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405344

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405344
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10011502
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG12100.019.0652221
GTCCTAG22700.016.5330541
GTATTAG19450.015.9321851
GTTCTAG26600.015.7917641
GTCCTAA19500.015.5381941
GTACTAA19250.015.0245341
TCTATAC20300.013.9086653
GTCTTAG21800.013.8988421
GTATAGG16450.013.6050421
GTATAAG20400.013.333661
GTATAGA21250.013.2864031
CTGTACT51300.013.15560154
GTATTAT29250.013.0662081
GGTCTAG19850.013.0092781
GTGCTAA22400.012.7579981
TCTGTAC41750.012.618353
CGAATCG4651.546141E-1012.60974145
GTATATG28900.012.5096141
GTATTAA29800.012.4784291
TACTAGG13600.012.4054922
Produced by FastQC (version 0.11.5)