FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405347

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405347
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10151160
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG12300.017.0881821
GTATTAG19600.017.052451
GTCCTAG26150.015.8117781
GTGCTAG23750.015.5235591
GTCTTAG24550.015.2984051
GTATAGG15800.015.047461
GTTCTAG31350.014.7278181
GTTCTAA28900.014.54565051
GTGTTAG24350.014.4335231
GTCCTAA21400.014.3300391
GGTCTAA18250.013.9714031
GTATTAA29350.013.7356411
GTATATA33550.013.4539541
GTATAAG19500.013.07581
CGATCGT3751.6279955E-812.85374538
GGTCTAG19400.012.7879791
GTATATG29000.012.713261
GTCTAGA25250.012.5544481
GTACAGA43100.012.4714951
TCTAGAC22150.012.2782083
Produced by FastQC (version 0.11.5)