FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405348

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405348
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9937443
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG11850.018.8827231
GTCCTAG22850.016.1200791
GTATTAA31700.014.8775311
GTATTAG18850.014.7925561
GTTCTAG27650.014.5666691
TACGCGA1901.3072797E-414.4945922
GTGCTAG25250.014.4515371
GTTCTAA28750.013.7698611
GCCCTAG23600.013.419781
TCTGTAC40800.013.0781243
TCTATAC21650.013.0385683
GTATAAA36600.012.9797881
GTACTAA16750.012.947781
GTATATA30300.012.8382361
GTACAGG42500.012.7978721
GTGTAGG35300.012.6776341
GTATAAG19600.012.6457911
GGTCTAG19900.012.6281391
GTATAGG15000.012.6223741
GTATACG4104.1909516E-912.5943851
Produced by FastQC (version 0.11.5)