FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405349

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405349
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9937443
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAT1500.006340014718.8379570
GTCCTAG24850.017.1884231
GTATTAG18000.016.0748731
GTTCTAA26850.015.5232211
GTCCTAA21050.014.72757151
GTTCTAG28650.014.6681721
GTCTTAG20000.014.6396161
GTGCTAG23500.014.5113591
GTATATA31900.014.0376181
GTCTTAA26800.013.8812871
GGTCTAA19750.013.7784611
GTCTAGA21000.013.4504031
GGTCTAG18200.013.4377861
GTATAGG16450.013.4015441
CGATCGT3609.19681E-913.388782538
GTACTAA19650.013.14738751
GTATTAA28300.013.0238111
GTACTAG11150.012.9752341
GTGTTAG26650.012.7961341
GTATATG27350.012.4686271
Produced by FastQC (version 0.11.5)