FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7958522
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAA11350.019.3786131
GTATTAG15550.017.4596391
GTACTAG8650.015.4948381
GTGCTAG21350.015.4529681
GTCTTAA20200.014.9716561
GTTCTAG22050.014.9623991
GTATTAA19800.014.5798361
GTGTTAG19000.014.10845851
TCTATAC16100.013.8512973
CTATACT17650.013.4036564
GTCCTAA15900.013.4008741
GTATATG22100.013.373491
CTAGTAC7950.013.3694484
GTCCTAG19650.013.2919771
GTATTAC13500.013.2375661
GCCCTAG19800.013.191281
GTATAGA16000.013.1023251
GTCTTAG18850.012.762191
GTCCTAC17900.012.6715421
CGATCGT3255.468228E-712.65632438
Produced by FastQC (version 0.11.5)