FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405352

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405352
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7908919
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAA22550.015.9591551
GTTCTAG23250.015.9209131
GTATTAG14050.015.8563841
GTACTAA14750.015.5686121
GTTCTAA22750.014.6136091
GTCCTAG18700.014.4794681
GTACTAG9050.014.3913711
GTATATA23000.013.8586921
GTCTTAG17150.013.5897661
GTCTATA12650.013.2761671
GTACTAT13200.013.2422991
GTGCTAG17300.013.0757011
GTCCTAT15200.012.8528191
ATAGTAC13450.012.7414133
TAGTACT14650.012.6336474
GTACAGA37450.012.629741
GTACAGG35100.012.4012021
TCTAGAC18050.012.3425823
CTAGACG4451.1459633E-912.3234584
CTAGACT16200.012.271164
Produced by FastQC (version 0.11.5)