FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405353

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405353
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7908919
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG9500.018.4571651
GTATTAG15650.017.3552631
GTCTTAG16350.017.2430691
GCGCTAG2652.391971E-916.866151
GTACTAA15350.016.126591
GTGCTAG18250.015.6362891
CGATCGT2705.67361E-815.23917538
GTTCTAG22000.015.1588941
GTCCTAG18900.015.0985331
GTTCTAA21150.014.7927691
GCTCTAG21300.014.5271821
GGTCTAA14450.014.27584651
GTATTAA20800.014.0499271
CCTATAC11350.013.9100643
GTACATA17150.013.6321151
GTCCTAA18250.013.564011
GGTCTAG16600.013.4624381
GTGCTAA17300.013.3151841
GTCCTAT16850.013.2626991
CTATACT17600.013.2501224
Produced by FastQC (version 0.11.5)