FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405356

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405356
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7693696
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG15650.018.1808511
GTATTAA20650.016.2688371
GTCCTAG18350.016.0661891
GTTCTAA20950.014.89045051
GTACTAG8650.014.6634471
GTTCTAG21300.014.6457711
GGTCTAA15500.014.5969711
GTATTAT19100.013.9994741
GTATAAG14800.013.6659811
GCTCTAG23650.013.3354341
GTGTATA18300.013.1128611
GTCTTAG15950.013.1105111
GGTCTAG14750.013.0150691
TCTAGAC16800.012.8552873
GTGCTAG19500.012.6575131
TCTAGGC22750.012.6575123
GTATATA22150.012.2265471
GCGTTAA2852.5165786E-512.0283381
GTATATG20450.011.9018791
GTACAGG34300.011.8933261
Produced by FastQC (version 0.11.5)