FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405357

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405357
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7693696
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG13950.016.7257481
GTCCTAG19450.015.700781
GTACTAA14350.015.5421921
GTTCTAG19000.015.3502751
TCTAGAC15750.015.23914053
GGTCTAA14800.015.0696261
TACGCGT1400.00431150214.69230654
GTGCTAG17750.014.6914971
GTATAGG10350.014.5869051
GTCCTAA15300.014.3528911
GTATTAA21350.014.1428071
GTACTAG7350.014.0050531
CTAGCGT3455.3696567E-913.9115564
GTACAGA35550.013.609131
GCGTTAG3052.4963265E-713.4999531
GTTCTAA21200.013.433621
GTCTTAA20800.013.1970691
GCTCTAG22850.013.0642331
GGTCTAG13950.013.0362441
GTACAAG25600.013.0011751
Produced by FastQC (version 0.11.5)