FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405359

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405359
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16355277
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG16650.016.8468721
GTACTAA30300.016.5476741
GTCCTAG39200.016.3684881
GTTCTAG42150.015.7219991
GTATTAG25850.015.5984281
GTATTAA43500.015.5564881
GGTCTAA29200.015.48996451
TCTATAC32750.014.8617573
GTGCTAG39900.014.2359231
GTCTTAG35000.014.02502251
GTGCTAA38400.013.9702371
GTATATA45800.013.7800421
GTCCTAA31200.013.7103581
GGTCTAG28900.013.5882561
GTACAGG73150.013.5169381
GTTCTAA45500.013.4855991
GCTCTAG46600.013.3929931
GTATAGG25450.013.2259541
GTACTAT27850.013.2192751
TAGTGCG8250.013.15077257
Produced by FastQC (version 0.11.5)