FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405360

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405360
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16102715
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG28500.017.440111
GTATTAA41400.016.4869371
GTCTTAG34400.015.8734681
GTCCTAG38000.015.7513671
GCTCTAG45750.015.684421
GTACTAA29000.014.6047141
GTTCTAG41250.014.5103481
GTATATA47250.013.7789721
GTGCTAG35050.013.6816421
GTATATG41550.013.6473921
GTACTAG18250.013.6176191
TACGCGA3951.875378E-913.2924152
TCTATAC32500.013.1398873
GTACAGG72200.013.0897771
GTATAGG24150.013.0446091
GGTCTAA28200.013.0330451
GTCCTAT33400.012.6807391
CTATACT36200.012.6672754
GTGCTAA37300.012.4809741
ATCTCCG143100.012.4491470
Produced by FastQC (version 0.11.5)