FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405361

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405361
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16102715
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG17150.017.3788171
GTATTAG28900.016.8648451
GTGCTAG36600.016.4783151
GTATTAA47150.015.9146651
GTCTTAG34950.015.851681
GTCCTAG38950.015.844211
GTTCTAG43850.015.75295451
GTCCTAA29650.015.4921621
GTACTAA27750.015.4156641
GCGATCG7500.014.93257837
CGATCGT7500.014.93257838
GCTCTAG44750.014.574221
GCGCTAG6350.014.3570441
GTGCGTA8100.014.2854621
GGTCTAG29550.014.1206551
GTACAGG75200.013.9884251
GTGCTAA39000.013.935821
GTATATG43450.013.4769651
GTACATA40750.013.4233961
TCTGACG116950.013.16739670
Produced by FastQC (version 0.11.5)