FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405363

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405363
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15857279
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG37750.017.347411
GTACTAG19500.016.701611
GTCCTAA31100.016.4400251
GTATTAG28350.016.3053781
GTGCTAG36800.015.2258721
GTACTAA28000.015.133431
GTTCTAG45300.014.9200091
GTATATA44150.014.5154461
GGTCTAG30400.014.5146631
GTATTAA45200.014.3332041
GTATAGG25850.014.2245571
GTCTTAA39750.014.0959011
GCTCTAG42850.013.5664821
GGTCTAA28750.013.5205751
GTCTTAG35100.013.4690411
TATACTG43500.013.2751765
GTTCTAA43000.013.2748361
ATTAGAC30100.013.0225423
GTCCTAT35300.012.9959071
CTATACT33650.012.79284
Produced by FastQC (version 0.11.5)