FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405364

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405364
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15830533
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG28400.017.501011
GTACTAG18450.017.0741561
GTTCTAG39550.016.5496251
GTATATA46250.015.665771
GTCTTAG33950.015.5679071
GTCCTAG37500.015.4942261
GTACTAA28400.015.0360791
GTGCTAG34950.014.9221771
GTATTAA42900.014.6045711
GTATAAG27350.014.4615291
GTCCTAA29950.014.0241811
GGTCTAG27900.013.8000781
CGAATCG8200.013.6584629
CTAGTAC16800.013.5425773
GTCTTAA38050.013.5224621
GTACAGG71450.013.079831
GTTCTAA43150.012.8164981
GTATAGG24950.012.6259781
GGATTAG33000.012.621941
TCTAGAC33100.012.5839273
Produced by FastQC (version 0.11.5)